Pc synthase
Splet02. mar. 2015 · PCS <1> [1] γ-glutamylcysteine dipeptidyl transpeptidase phytochelatin synthase CAS registry number 125390-02-5 Keywords Arabidopsis Thaliana Saccharomyces Cerevisiae Caenorhabditis Elegans Reaction Type Lycopersicon Esculentum These keywords were added by machine and not by the authors. SpletThe PC synthase pathway (purple), methylation pathway (orange), CDP-choline pathway (blue), and acyltransferase pathway (green) are displayed with the corresponding …
Pc synthase
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Spletjuncea PC synthase cDNA fragment obtained by 5¢ RACE-PCR. A biotinylated probe was synthesized with the following primers: sense 5¢-TGGCTCACTGTTCTGGAGC-3¢, antisense 5¢-CAAGACGAG- Splet06. okt. 2000 · Specifically, given that PC synthase is a dipeptidyltranspeptidase (1, 2, 10), the kinetics of heavy metal-dependent PC 2 synthesis from GSH implicate the formation, …
SpletReduced glutathione (GSH) serves as a precursor for PCs that are synthesized non-translationally by transpeptidation reaction catalyzed by the enzyme phytochelatin … Splet28. apr. 2006 · PC synthase is activated both in vivo and in vitro by a variety of heavy metal ions, including Cd 2+, Ag +, Cu 2+, Au +, Zn 2+, Fe 2+, Hg 2+ and Pb 2+. In crude protein extracts from tomato cells the enzyme has an apparent K m of 7.7 m M for GSH in the presence of 0.5 m M Cd 2+, and exhibits maximum activity at pH 8.0 and 35°C.
Splet30. nov. 2024 · The gene encoding PS synthase from M. janaschii (MjPSS) was cloned into a plasmid vector (pSKB2LNB) containing an N-terminal His 6-Tag and a protease recognition site for PreScission cleavage. SpletA, SM synthase activity assay, using NBD-Cer and PC as two substrates. B, PC-PLC activity assay, using 14C-PC as a substrate. +, bacterial PLC (20 ng) was used as a positive control; -, blank with no samples; PC, phosphatidylcholine; SMS, sphingomyelin synthase; PC-PLC, PC-phospholipase C. View Large Image Download Hi-res image Download (PPT)
SpletMeasurement of PCs, S-Alkyl-PCs, and PC Synthase Activity—PC synthase activity was assayed in reaction media (200 mM HEPES-BTP buffer, pH 8.0) containing purified …
SpletPCs are enzymatically synthesized peptides, whereas MTs are gene-encoded polypeptides. Recently, genes encoding the enzyme PC synthase have been identified in plants and … aurelion sol vs yasuoSplet28. okt. 2024 · The PC synthase pathway is exclusive to bacteria. Here, PC is synthesized via the condensation of CDP-DAG with choline by the PC synthase (Pcs). Pcs enzymes … galfusz norbertSplet04. apr. 2009 · The mutant PC synthase had a lower PC synthesis ability than the wild-type enzyme. Further, oxidative conditions severely damaged mutant PC synthase whilst the wild-type enzyme suffered less damage, suggesting that the Cys-rich region of PC synthase may play an important role in anti-oxidation activity. Although the C-terminal of PC … galga menti településekSplet06. okt. 2000 · The dependence of phytochelatin synthase (γ-glutamylcysteine dipeptidyltranspeptidase (PCS), EC 2.3.2.15) on heavy metals for activity has invariably … galga folyóSplet04. avg. 2024 · The brucellae are facultative intracellular bacteria with a cell envelope rich in phosphatidylcholine (PC). PC is abundant in eukaryotes but rare in prokaryotes, and it has been proposed that Brucella uses PC to mimic eukaryotic-like features and avoid innate immune responses in the host. Two PC synthesis pathways are known in prokaryotes: … galga egészségműhelySplet10. apr. 2024 · The production of phytylated chlorophyll a (Chl aP) in Rhodobacter sphaeroides, which uses phytylated bacteriochlorophyll a (BChl aP), is the first step in expanding the light absorption spectra. Unlike the chlorophyll synthase (ChlG) of the Synechocystis sp. PCC6803, ChlGs of angiosperms, including Arabidopsis thaliana, … aurelion vs yasuoSplet28. okt. 2024 · The PC synthase pathway is exclusive to bacteria. Here, PC is synthesized via the condensation of CDP-DAG with choline by the PC synthase (Pcs). Pcs enzymes are integral membrane proteins that belong to the superfamily of CDP-alcohol phosphotransferases. aurelisonline