2024 Pax7-creer小鼠

Pax7-creer小鼠

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August undefined, 2024

Splet四环素诱导型 tetO-Cre 将四环素调控系统与Cre-loxP系统相结合，一般需要两种转基因小鼠交配使用：一种是由四环素响应启动子元件（TRE，也叫 tetO ）控制的 Cre 工具鼠（TRE-Cre，也叫 tetO-Cre）。另一种是由组织特异性启动子驱动的表达四环素转录活化因子 rtTA 或 tTA 的小鼠。 tetO 本身缺乏启动子活性，无法独立驱动下游基因的表达。只有当具有 … SpletPAX7 品系描述将IRES-iCre插入到小鼠Pax7基因终止密码子处。 Pax7 在肌肉祖细胞表达，Pax7 对于生肌祖细胞的生肌潜能、存活和增殖至关重要。当Pax7-IRES-Cre与含有 loxP …

造血系统常用Cre转基因小鼠及其应用 - ResearchGate

Splet21. jul. 2024 · Pdgfra-creER转基因小鼠是由Pdgf receptor a的启动子驱动，主要在呼吸系统特异地表达creER重组酶。而creER会在他莫昔芬的诱导下产生活性。即可以实现时间和 … Splet图1. Cre-Lox基因重组系统工作原理[2] 利用Cre-Lox系统如何制备条件性敲除小鼠. Cre-Lox系统的优势在于可以利用组织特异性启动子特异性控制Cre在特定组织的表达，以实现在特 … freight ppd \\u0026 add

Nature 阻断β-AR可诱导新型米色脂肪形成 - 简书

Splet一、品系介绍： Cre-ERT2小鼠是一类含有雌激素受体（estrogen receptor，ER）的配体结合区突变体（ERT）与Cre重组酶的融合蛋白表达的小鼠。 Cre-ERT2在无Tamoxifen诱导的 … Splet期即开始表达。Berquam．Vrieze等的研究认为， Vav—Cre在HSC中开始表达。Stan等检测到利用Vav—Cre小鼠可以介导100％的造血细胞发生基 SpletPax7CreER/+-tdTomatofl/+ (Pax7-tdT) mice were generated by crossing male homozygous Pax7CreERT2 mice (Murphy et al., 2011), purchased from Jackson Laboratory, Bar Harbor, ME, with female R26LSL-tdTomato mice (Madisen et al., 2010), also purchased from Jackson Laboratory, as previously described by our laboratory (Murach et al., 2024). freight ppv

Inducible depletion of satellite cells in adult, sedentary ... - Nature

Pax7-Cre小鼠--兰博模型LABMODEL 兰博动物LABANIMAL 兰博细 …

Splet010530 STOCK Pax7 tm1(cre)Mrc /J This Pax7 knock-in strain may be useful in studies of alveolar rhabdomyosarcoma and embryonic myogenesis. If you need help with your … Splet15. dec. 2014 · The Pax7 CreER strain was generated by placing an ires-CreERTM-FRT-Neo-FRT cassette into the ClaI site of the Pax7 gene 3′-UTR following the stop codon in exon 9 (ref. 40). Mice are on a mixed ... fast easy dinner for twoSpletSC-derived-cell contributions to skeletal muscle maintenance were examined in castrated Pax7 CreER/+; ROSA26 mTmG/+ mice. SCs were depleted in Pax7 CreER/+; ROSA26 DTA/+ mice to ascertain the consequences of SC ablation in sham and castrated skeletal muscles. Confocal immunofluorescence analysis of neuromuscular junctions (NMJs), and … fast easy dinner ideas with ground beef

"Splet21. jan. 2024 · 先说 Xcr1^ {iCre} 老鼠。它指的是在老鼠的基因组中插入了一个连接有mCherry的Cre片段，该片段在XCr1启动子的控制之下，只会在cDC1细胞表达。表达时，mCherry和Cre会被内源酶切开，mCherry用来指示表达的细胞，而Cre产生作用。 Cre产生什么作用呢？它会识别loxP-flanked序列，将序列之后的基因敲除，因而称作conditional … " - Pax7-creer小鼠

Pax7-creer小鼠

In vivo partial reprogramming of myofibers promotes muscle

SpletLimitations of the Pax7-creERT2 transgene for driving deletion of Nf1 in adult mouse muscle Neurofibromatosis Type 1 (NF1) is an autosomal dominant genetic disorder that … Splet在小鼠中， Cre-lox系统最初被用于在特定细胞群中打开基因的表达。. 条件性基因表达所需要的Flox小鼠，一般带有一个沉默的转基因，也就是说在靶基因与启动子之间有一个“ 终 …

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SpletPax7CreERsystem in postnatal satellite cells delayed injury-in- ducedmusclerepairduetoareducednumberofmyoblastsdur- ing regeneration. Analysis of satellite cell dynamics on myofi- bers confirmed that HIF1 /2 the proline residue and subjected to ubiquitination and pro-dKO myoblasts exhibit reduced Splet01. maj 2006 · Skeletal muscle growth and regeneration are attributed to satellite cells - muscle stem cells resident beneath the basal lamina that surrounds each myofibre. Quiescent satellite cells express the transcription factor Pax7 and when activated, coexpress Pax7 with MyoD. Most then proliferate, downregul …

SpletPax7 CreERT2 mice efficiently label satellite cells. (A) Pax7 CreERT2 targeting strategy. (B-D) In myofibers isolated from uninjured tibialis anterior muscles (TAs) from Pax7 CreERT2/+... Splet14. mar. 2024 · View Tg(Pax7-EGFP)15Tajb allele: origin, molecular description, gene associations, and references.

Splet21. jul. 2024 · Pdgfra-creER 转基因小鼠是由Pdgf receptor a的启动子驱动，主要在呼吸系统特异地表达 creER重组酶。而creER会在他莫昔芬的诱导下产生活性。即可以实现时间和空间皆可控的cre表达，一般用于配合Flox条件性敲除小鼠实现组织特异性和时间特异性的基因敲除。转基因小鼠的构建一般不使用定点插入或重组，使用随机插入的策略比较常见 … Splet23. nov. 2024 · We subjected Pax7-DTA mice to 4 and 8 weeks of PoWeR and performed immunohistochemistry (IHC) on the soleus (oxidative phenotype) and plantaris (mixed oxidative and glycolytic phenotype) of vehicle- (SC+) and tamoxifen-treated (SC−) mice and whole-muscle RNA-sequencing (RNA-seq) on the soleus.

Splet25. jul. 2014 · Tg (Pdx1-cre)PBMga 小鼠是将1kb 增强子区域和Hsp68 启动子融合驱动cre，此小鼠在所有内分泌细胞中均有cre 活性。二、insulin-cre 1.基因名称：小鼠和大鼠的insulin 基因不同于其他物种，有两个拷贝，ins1 和ins2，在卵黄囊中仅ins1 表达，在胰腺中ins1 和ins2 均表达。在某些神经类细胞中ins2 也有表达，成体细胞中ins1 表达更特异。 …

SpletIntroduction. The primary muscle stem cell population is Pax7+ satellite cells. 1 Throughout postnatal development, these cells fuse to growing muscle fibers and contribute new myonuclei. 2 When satellite cells are depleted using a genetic approach in prepubertal murine muscle, muscle fibers are smaller by adulthood, 3 and when depleted in young … freight prepaid collect คือSplet所谓 reporter鼠，就是敲入了报告基因（reporter gene）的鼠。常见的有 TdTomato鼠、mT/mG鼠、LacZ鼠、彩虹鼠等，他们的重要作用是对各种特异性表达的Cre工具鼠进行 … freight ppd and allowedSpletCre-loxP重组系统，即对一段特定的DNA序列进行定位并用Cre重组酶对其进行剪接，由Cre重组酶和loxP位点两部分组成。. 其中Cre蛋白，最初由“导致重组（Cause … freight ppd/addSplet39 Pax7 ZsG mice were generated by crossing Pax7-CreERT2 with ROSA26-LoxP-STOP-loxP-ZsGreen mice (Fig. 1a). The primers and PCR conditions for genotyping are detailed … freight prepaid atSplet10. jul. 2024 · Pax7 CreER-R26R DTA mice were treated for 5 days with vehicle (satellite cell-replete, SC+) or tamoxifen (satellite cell-depleted, SC-) at 2 months (young) and 4 months (mature) of age. Following a 2-week washout, mice were subjected to sham surgery or 10 day synergist ablation overload of the plantaris ( n = 6–9 per group). freight prepaid and charged backSpletPAX7 品系描述将IRES-Cre-ERT2插入到小鼠Pax7基因终止密码子处。 Pax7 在肌肉祖细胞表达，Pax7 对于生肌祖细胞的生肌潜能、存活和增殖至关重要。 Pax7-IRES-Cre与含有 … fast easy dinner ideas for kidsSplet购买pax7小鼠单克隆抗体[pax7497](ab199010)，pax7抗体经wb验证，可与人样本反应。12篇文献引用，2个独立用户反馈。产品出库一年都在质保范围内。中国现货速达。 freight predictive analytics