Web12 apr 2024 · After transfection and hydrogen peroxide stimulation, 10 μl Cell Counting Kit 8 (CCK8, Dojindo, Japan) was added to each well for 2 h. Cell viability was measured at absorbance at 450 nm with a microplate reader (Bio-Rad Laboratories, Benicia, CA, USA). Concentration of cell supernatants. The treated cell supernatant was collected. WebAddress: 2025-5 Tabaru Mashiki-machi, Kamimashiki-gun, Kumamoto 861-2202, Japan. +81- (96)-286-1515 Fax: +81- (96)-286-1525 . Profile: Dojindo Laboratories deals with …
Dojindo Laboratories - Kumamoto, Japan @ ChemicalRegister.com
http://www.dojindo.eu.com/TechnicalManual/Manual_CK04.pdf Web6 apr 2024 · Anchorage-dependent cell viability was evaluated with a CCK-8 kit (Dojindo Laboratories) according to the manufacturer’s instructions. For the colony formation assay, stably transfected 786-O and Caki-1 cells or parental 786-O and Caki-1 cancer cells were plated in six dishes in triplicate at 2000 cells per well to adhere overnight and then … gold jewelry marks identification
同仁化学/DOJINDO LABORATORIES
Web31 mar 2024 · The Japanese address format can be quite confusing for many foreigners living in Japan. In this article, you will learn how to read, write, say, and understand … WebThus, the measurement of the amount of released LDH from cells is one of the major methods to assess the cell death. Since Dojindo’s Cytotoxicity LDH Assay Kit-WST neither reflects the activity of living cells nor is … Webby utilizing Dojindo’s highly water-soluble tetrazolium salt. WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator, as shown in Figure 1. CCK-8 is a one-bottle solution; no premixing of components gold jewelry made in italy